Table 2.
The in vitro antioxidant actions of BN 80933, Trolox, and BHT
| Antioxidant | IC50, μM |
|---|---|
| BN 80933 | 0.29 (0.22–0.38) |
| Trolox | 39.4 (31–49) |
| BHT | 4.9 (2.2–11) |
Rat brain homogenate (0.5 ml) was incubated for 15 min at 37°C with test compound or vehicle (10 μl). Lipid peroxidation was initiated by the addition of 50 μl of 1 mM FeCl2, 1 mM EDTA, and 4 mM ascorbic acid. After 30-min incubation at 37°C, the reaction was stopped by adding 50 μl of 0.2% BHT. Lipid peroxidation was determined by the LPO method (Bioxytech, Gagny, France) using a chromogenic reagent, N-methyl-2-phenylindole, which reacts with malonaldehyde at 45°C to yield a stable chromophore with maximal absorbance at a wavelength of 586 nm. Results represent the mean of two or three determinations, with 95% confidence limits in parentheses.