Table 3.

Dose–response of BN 80933 in rat model of focal ischemia

Treatment	Infarct volume, mm3
	0.1 mg/kg	0.3 mg/kg	1 mg/kg	10 mg/kg
Vehicle	295.36 ± 31.99 (8)	311.70 ± 34.05 (5)	269.81 ± 32.37 (9)	295.36 ± 31.99 (8)
BN 80933	299.56 ± 14.27 (8)	93.90 ± 31.94** (7)	86.77 ± 29.94** (7)	92.23 ± 25.66*** (8)

Dose–response study of BN 80933 treatment on the infarct volume and on the total neurologic score after 2 h of MCAO. Rats were subjected to i.v. administration of vehicle or the indicated dose of BN 80933 at 4 and 24 h after onset of ischemia. The infarct volume was assessed 48 h after MCAO; six coronal sections of the brain were stained with 2,3,5-triphenyltetrazolium chloride for 20 min at room temperature and the infarct size was determined by an indirect method for calculating infarct volume (40), using an image analysis system. Results are mean ± SEM with the number of measurements in parentheses. ∗∗, P < 0.01 and ∗∗∗, P < 0.001 for comparison between vehicle-treated group and BN 80933-treated group.