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. 2006 Dec 8;8(1):70–76. doi: 10.1038/sj.embor.7400849

Figure 3.

Figure 3

Identification of c-MYC targets involved in carcinoma cell proliferation. (A–E) WNT5A gene repression contributes to the mitogenic function of c-MYC in MDA-MB-231 and HeLa cells. (A) WNT5A gene expression, normalized by 18S ribosomal RNA, was quantified by real-time reverse transcription–PCR analysis in control, c-MYCKD (knockdown), MIZ-1KD or c-MYC/MIZ-1DKD (double knockdown) cells. (B) Chromatin immunoprecipitations (ChIPs) were carried out with antibodies against c-MYC and MIZ-1, or a pre-immune serum (Ctrl). ChIP was evaluated using quantitative real-time PCR analysis, with primers in the WNT5A gene regulatory region. (C–E) MDA-MB-231 and HeLa cells were treated (C) or transfected with the indicated expression vectors (D) or small interfering RNA (E) and counted 6 days later.