Figure 3.
C. elegans Rabenosyn-5 mutant shows a phenocopy of the vps-45 mutant in various endocytic defects. (A) Yeast two-hybrid assay. (B) Receptor-mediated endocytosis-defective (Rme) and coelomocyte uptake (Cup) phenotypes in Rabenosyn-5 (rabs-5) mutant. (C) Individual coelomocytes from animals expressing RME-8/GFP. (D) Quantitative analysis of (C). Error bars indicate s.e.m. *P<0.001. Scale bars, 20 μm (B, upper), 50 μm (B, lower) and 5 μm (C). DIC, differential interference contrast; GFP, green fluorescent protein.