Figure 2.
SGO2 and RAD21, but not REC8, show a complex arrangement at the inner domain of metaphase I centromeres and change their relative distributions during telophase I. Double immunolabellings of SGO2 (green) with kinetochores (ACA; pseudocoloured in pink; A–E′), RAD21 (red; F–J′) or REC8 (blue; K–O′) in metaphase I (A–C′,F–H′,K–M′) and telophase I (D–E′,I–J′,N–O′) centromeres. (A–A″,F–F″,K–K″) Side views of autosomal metaphase I bivalents. (B,B′,G,G′,L,L′) Side views of metaphase I centromeres. (C,C′,H,H′,M,M′) Top views of metaphase I centromeres. (D,D′,I,I′,N,N′) Side views and (E,E′,J,J′,O,O′) top views of telophase I centromeres. ACA, anti-centromere autoantibody.