Figure 5. Hypoxia stimulates K_Ca currents and proliferation of IGR39 and of primary human melanoma cells.

A, mean K_Ca currents, ratio of currents, current densities and cell capacitances for IGR39 cells (number of cell batches, N = 5) and primary human melanoma metastasis cells (N = 2). The open bars indicate control measurements and the grey bars indicate measurements after 24-h exposure to 100 μm CoCl₂. B, proliferation of IGR39 cells under normoxic (○) or hypoxic conditions (•) during a period of 48 h. Proliferation rates were determined as BrdU incorporation (N = 1) or MTT conversion (N = 4) and normalized to the respective normoxic controls. Mean values of all experiments are shown for the following conditions: control (−), presence of 100 nm apamin (A), 100 nm ChTX (C) or 100 nm of each blocker (AC). C, mean proliferation rates of primary human melanoma cells measured by BrdU incorporation (N = 3). All symbols are the same as in B.