Table 1.
Parameters of rectification in Kir2.1–3 channels in high extracellular K+ at 300 μM spermine
| Z0 | Z1 | V01/2 | V11/2 | n | |
|---|---|---|---|---|---|
| Kir2.1 | 5.0 ± 0.2 | 1.4 ± 0.1 | −23.4 ± 1.0 | −64.6 ± 4.3 | 7 |
| Kir2.2 | 5.9 ± 0.2* | 1.5 ± 0.1 | −22.4 ± 0.3 | −75.5 ± 1.9 | 4 |
| Kir2.3 | 5.2 ± 0.1 | 1.7 ± 0.1 | −24.6 ± 0.4* | −52.2 ± 0.8** | 9† |
n is number of cells
Only 3 cells expressing Kir2.3 channels at a level suitable for reliable analysis are included. The remaining 6 cells expressed Kir2.3-YFP fusion channels. The presence of a YFP tag dramatically facilitates selection of highly expressing cells. There was no measurable difference in any parameter between the two channel constructs, and thus the data were pooled together
P < 0.05
P < 0.01 compared to Kir 2.1