Table 4.
Parameters of rectification in mutant Kir2.1 channels at 30 μM spermine
| Z0 | V01/2 | Z2 | V21/2 | n | |
|---|---|---|---|---|---|
| WT Kir2.1 | 5.3 ± 0.1 | −8.5 ± 1.0 | 3.3 ± 1.0 | 24.8 ± 1.3 | 3 |
| E299S | 2.8 ± 0.2 | 1.1 ± 1.9 | 1.1 ± 0.3 | 99.3 ± 3.0** | 5 |
| F254A | 5.2 ± 0.1 | −7.1 ± 0.1 | 2.1 ± 0.1 | 45.6 ± 2.5** | 3 |
| S256I | 4.6 ± 0.1 | −7.9 ± 0.3 | 3.7 ± 0.2 | 24.8 ± 0.1 | 2 |
| S256K | 5.6 ± 0.2 | −7.3 ± 0.4 | 3.6 ± 0.7 | 21.3 ± 2.1 | 2 |
**
Statistical significance relative to wild-type (WT) Kir2.1 channels (P < 0.01). V21/2 potential could not be determined with certainty in E224G channels at 30 μm spermine, and thus data were not included. Experiments for Kir2.1 presented in this table are independent of those in Table 2.