Table 1.

Kinetics of wild-type and mutant Kir6.2 channels with and without SUR1

	Open time			Closed time			Burst duration (ms)	Openings per burst
	Short open time τo1 (ms)	Long open time τo2 (ms)	percent o2	Short closed time τc1 (ms)	Long closed time τc2 (ms)	percent o2
Kir6.2 C166A without	0.71 ± 0.02	2.2 ± 0.15	∼100%	0.6 ± 0.1	15 ± 4	2.5 ± 1.5%	432 ± 138	190 ± 90
SUR1		2.5 ± 0.12	99 ± 0.5%	0.61 ± 0.04	15.5 ± 0.04	2 ± 0.5%	354 ± 55
Kir6.2C166A/	0.74 ± 0.2	2.5 ± 0.5	21 ± 9%	0.8 ± 0.2	18 ± 5 17 ± 0.3	73 ± 13%	11 ± 4	4.2 ± 1
R176E/R177E + SUR1	0.77 ± 0.02	2.2 ± 0.09	27 ± 0.6%	0.62 ± 0.08		76 ± 1.2%	5.5 ± 1.6
Kir6.2R54E + SUR1	0.82 ± 0.2	2.35 ± 0.2	42 ± 11%	0.67 ± 0.9	23 ± 7.2	6.5 ± 2%	31 ± 15 44 ± 5	12 ± 2.4
	0.67 ± 0.01	2.3 ± 0.14	78 ± 1%	0.61 ± 0.01	22 ± 0.03	26 ± 1.4%
Kir6.2 without SUR1	0.7 ± 0.15	2.1 ± 0.1	28 ± 7%	0.7 ± 0.2	13 ± 4	76 ± 15%	5.6 ± 1.2	2.2 ± 0.8
	0.8 ± 0.02	2 ± 0.2	14 ± 0.04%	0.61 ± 0.01	11.5 ± 0.1	91 ± 0.02%	6.2 ± 0.7
Kir6.2 + SUR1	0.65 ± 0.1	2.6 ± 0.3	84 ± 12%	0.6 ± 0.1	14 ± 3 14.5 ± 0.5	21 ± 14%	51 ± 25 55 ± 6	20 ± 6
	0.75 ± 0.03	2.35 ± 0.03	94 ± 2%	0.61 ± 0.42		11 ± 3%

In each cell the numbers in italic are fitted values using the kinetic model in Fig. 10. The other numbers are experimental values, reflecting at least 3 experiments for each case.