Table 1.
Primer sequences for real-time RT-PCR (Tm: annealing temperature)
Gene | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) | PCR product size (bp) | Tm (°C) |
---|---|---|---|---|
β-actin | AGAGGGAAATCGTGCGTGAC | CAATAGTGATGACCTGGCCGT | 138 | 57 |
Car1 | TTGATGACAGTAGCAACC | CCAGTGAACTAAGTGAAG | 161 | 51 |
Car2 | CAAGCACAACGGACCAGA | ATGAGCAGAGGCTGTAGG | 122 | 56 |
Car3 | GCTCTGCTAAGACCATCC | ATTGGCGAAGTCGGTAGG | 160 | 54 |
Car4 | CTC CTT CTT GCT CTG CTG | GAC TGC TGA TTC TCC TTA | 145 | 55 |
Car9 | CTGAAGACAGGATGGAGAAG | GCAGAGTGCGGCAGAATG | 221 | 57 |
Car12 | CCTATGTTGGTCCTGCTG | CGTTGTAACCTTGGAACTG | 143 | 53 |
Car13 | AATACGACTCCTCACTCC | TGCCGCAACCTGTAGTTC | 116 | 52 |
Car14 | TGTTGTTCTTCGCTCTCCTG | CACTGTCTGTCTGGATATTG | 161 | 53 |