TABLE 3.

Transition state and bound state properties of barnase-barstar complex

r0 (Å)	6
cosθ0	−1
(Å)	4.9 ± 0.5
	0.89 ± 0.08
	3.2 ± 2.5
	0.92 ± 0.05
	2.8 ± 1.7
	0.01 ± 0.31
Nc;max	30
	14
fc	0.101 × 10−3
fb	0.059
〈r2〉′b (Å2)	19.5
𝒱b (10−3 Å3)	8.75
Interface area (Å2)*	797
Ka (1012 M−1)†	8

^*Taken as half of the buried solvent-accessible area calculated with a 1.4 Å probe radius.

^†Schreiber and Fersht (24). The value listed is for an ionic strength of 125 mM.