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. Author manuscript; available in PMC: 2007 Feb 9.
Published in final edited form as: J Neuroimmunol. 2006 Dec 6;182(1-2):226–231. doi: 10.1016/j.jneuroim.2006.10.019

Fig. 3.

Fig. 3

Sorted Lin−CD117+CD34+ and Lin−CD117+CD34− BM cells from young ALS2+/+ and ALS2−/− mice were cultured in DMEM in 12-well polystyrene tissue culture plates at 3000 cells/well in the presence of 3 ng/mL IL-3 and 5 ng/mL IL-6 with or without 25 ng/mL SCF. Cells were cultured at 37°C with 5% CO2for 7 days. Harvested cells were counted under a light microscope to calculate fold of cell expansion defined as the ratio between the number of cells recovered and the number of cells used to initiate culture. Data presented as means with standard error bars from duplicate or triplicate culture wells for each cell type.