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. 2007 Jan 25;7:17. doi: 10.1186/1471-2407-7-17

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Copyright © 2007 Chiappetta et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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RT-PCR analysis of the fra-1 gene expression in paraffin-embedded tissues. The mRNA was extracted from paraffin-embedded tissues and amplified by RT-PCR using fra-1 specific primers as specified under "Material and Methods". The cDNAs were co-amplified with gapdh gene as an internal control. Sources of RNA: lane 1, normal breast tissue; lanes 2–4, breast dysplastic lesions; lanes 5–7, breast fibroadenomas; lanes 8–12, breast carcinomas; lane 13: water control. Molecular markers are indicated.