Table 1.
Primer sequences used to amplify intergenic fragments for subsequent cloning
| Construct* | Primer sequence | |
| (Forward) | (Reverse) | |
| pGL3-Basic constructs | ||
| 1. SERPINI1 (1-851) | 5'-ATAGATCTACTCCGGCGACGCCGGA-3' | 5'-ATAAGCTTGTCCAGACTGCGCCTCT-3' |
| 2. SERPINI1 (1-175) | 5'-ATAGATCTACTCCGGCGACGCCGGA-3' | 5'-ATAAGCTTTCGCCTCAGGATCCCCC-3' |
| 3. SERPINI1 (176-851) | 5'-CGAGATCTTGGGTGGTGACACTTCT-3' | 5'-ATAAGCTTGTCCAGACTGCGCCTCT-3' |
| 4. SERPINI1 (474-851) | 5'-AGAGATCTGTGTGGGGACTTGCACT-3' | 5'-ATAAGCTTGTCCAGACTGCGCCTCT-3' |
| 5. SERPINI1 (711-851) | 5'-ATAGATCTCAGGGCAGCTGCTTTCC-3' | 5'-ATAAGCTTGTCCAGACTGCGCCTCT-3' |
| 6. PDCD10 (851-1) | 5'-ATAGATCTGTCCAGACTGCGCCTCT-3' | 5'-ATAAGCTTACTCCGGCGACGCCGGA-3' |
| 7. PDCD10 (710-1) | 5'-TCAGATCTGTACCTTTCCGCTCCCT-3' | 5'-ATAAGCTTACTCCGGCGACGCCGGA-3' |
| 8. PDCD10 (473-1) | 5'-ATAGATCTCAAGCCCTCCCTTCACC-3' | 5'-ATAAGCTTACTCCGGCGACGCCGGA-3' |
| 9. PDCD10 (175-1) | 5'-ATAGATCTTCGCCTCAGGATCCCCC-3 | 5'-ATAAGCTTACTCCGGCGACGCCGGA-3' |
| 10. PDCD10 (107-1) | 5'-ATAGATCTGCCGGCGAGCGGGACTA-3' | 5'-ATAAGCTTACTCCGGCGACGCCGGA-3' |
| 11. PDCD10 (175-108) | 5'-ATAGATCTTCGCCTCAGGATCCCCC-3' | 5'-CCAAGCTTGTTGTCCGCAATTCCTG-3' |
| 12. PDCD10 (851-176) | 5'-ATAGATCTGTCCAGACTGCGCCTCT-3' | 5'-CGAAGCTTTGGGTGGTGACACTTCT-3' |
| Heterologous promoter constructs | ||
| 1. SERPINI1 (176-851)/S | 5'-CCTGCTAGCTGGGTGGTGACACTTCTAG-3' | 5'-AAAGATCTGTCCAGACTGCGCCTCTG-3' |
| 2. PDCD10 (851-176)/S | 5'-TAAGCTAGCGTCCAGACTGCGCCTCTG-3' | 5'-CCTAGATCTTGGGTGGTGACACTTCTAG-3' |
| 3. PDCD10 (473-176)/S | 5'-TATGCTAGCCAAGCCCTCCCTTCACCTGG-3' | 5'-CCTAGATCTTGGGTGGTGACACTTCTAG-3' |
| 4. PDCD10 (710-474)/S | 5'-GCTGCTAGCGTACCTTTCCGCTCCCTG-3' | 5'-GAAGATCTGTGTGGGGACTTGCACTG-3' |
| 5. PDCD10 (851-711)/S | 5'-TAAGCTAGCGTCCAGACTGCGCCTCTG-3' | 5'-TTAGATCTCAGGGCAGCTGCTTTCCG-3' |
| 6. SERPINI1 (176-473)/C | 5'-CCTGCTAGCTGGGTGGTGACACTTCTAG-3' | 5'-CCAGATCTCAAGCCCTCCCTTCACCTGG-3' |
| 7. SERPINI1 (474-710)/C | 5'-GGAGCTAGCGTGTGGGGACTTGCA-3' | 5'-CTAGATCTGTACCTTTCCGCTCCCTG-3' |
| 8. SERPINI1 (711-851)/C | 5'-CTTGCTAGCCAGGGCAGCTGCTTTCCG-3' | 5'-AAAGATCTGTCCAGACTGCGCCTCTG-3' |
*Construct names with an "S" character at the end indicates that the PCR-amplified intergenic product was cloned into the upstream of SV40 minimal promoter. Construct names with a "C" character denotes that the amplified intergenic segment was cloned into the upstream of CMV promoter.