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. 2007 Feb 6;3(2):111–119. doi: 10.7150/ijbs.3.111

Figure 1.

Figure 1

Schematic diagram of the Elovl4 locus and targeting construct. The construct contains 5.5 kb and 3 kb of the 5' and 3' exon6 genomic sequence. A HA tag was inserted before the induced Y270X mutation. A floxed PGKNEO (Neo), positive selection marker, was also inserted into exon6. There is a HSV-TK counter selection cassette in the 5' end of the vector. The wild type band expected from EcoRI digestion is 13.9 kb, mutant band is 10.8 kb. The wild type and mutant bands expected from BamHI digestion are 7.7 and 5.0 kb, respectively.