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. 2007 Jan 5;3(2):91–99. doi: 10.7150/ijbs.3.91

Table 1.

Annealing oligos.

Component Amount Final
Ligation buffer (10X) 4 μl 1X
Oligo 1 (0.2 μg/μl) 18 μl 0.09 μg/μl
Oligo 2 (0.2 μg/μl) 18 μl 0.09 μg/μl
TOTAL 40 μl