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. 2007 Jan 5;3(2):91–99. doi: 10.7150/ijbs.3.91

Table 3.

EcoRI restriction digestion of the vector.

Component Amount Final
ApaI digested and blunted pBS/U6-neo vector 2.5μg
Buffer H (10X) 2.5 μl 1 X
EcoRI 1 μl (10 unit) 4 unit/μg of the vector
TOTAL (make up with dH2O 25 μl