Table 5.
Digestion of DNA using EcoRI + SalI.
| Component | Amount | Final |
|---|---|---|
| DNA from miniprep | 2.0 μl | |
| EcoRI | 1 μl | 10 unit/reaction |
| SalI | 1 μl | 10 unit/reaction |
| Buffer H | 2.5 μl (10X) | 1X |
| TOTAL | 25 μl |
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