Table 6.

PCR conditions for detecting the U6-neo transgene.

Cycle number	Denaturation	Annealing	Polymerization
1	2 minutes at 94°C
2-34	10 seconds at 94°C	20 seconds at 60°C	30 seconds at 72°C
35	10 seconds at 94°C	20 seconds at 60°C	10 minutes at 72°C