Figure 3.
Phosphorilated JNK1&2 protein levels (-fold of control) of JAR over 24 hours of exposure to hypoxia. The phosphorylated JNK 1&2 protein level was measured with Phospho-JNK1&2(pThr183/pTyr185) ELISA after 12 hours and 24 hours exposure to hypoxia. From the absorbance at 450nm, the phosphorylated JNK 1&2 protein level in comparison with the control was calculated according to the formula: Changes in the level of phosphorylated JNK 1&2 (-fold of control) = absorbance of treated cells / absorbance of control cells. All points were done duplicate X three times, and the results are average +/- SD. “* “means p<0.05, and “n.s”: means statistically not significant.