Table 1.

Altered protein expression of JAR cells induced by hypoxia in the apoptosis pathways using PowerBlot.

Protein	Confidence level	(-) Under (+)Over	Fold change	Protein	Confidence level	(-) Under (+)Over	Fold change
Bad	3	-	1.92	Bid	1	-	9.40
Bcl-x	3	+	2.39	Caspase-6	1	+	0/+
Caspase-3	3	+	4.96	Caspase-8	1	-	2.76
Caspase-7	3	+	2.16	Hsp90	1	-	2.32
Hsp70-64kD	3	+	1.85	Bax	0		1.25
Pan-JNK/SAPK1-50kD	3	-	2.21	FADD	0		1.15
PTEN	3	+	1.92
JNK-phospho-specific	2	+	2.29
Pan-JNK/SAPK1-43kD	2	-	2.17
BAG-1	1	+	0/+
Bcl-2	1	-	1.91

Fold change means a semiquantitative value that represents the general trend of protein changes for the experimental sample to control detected by using the Odyssey Infrared Imaging System. (+) means an increase of signal intensity, and (-) means a decrease of signal intensity after exposure to hypoxia.

Confidence levels are defined as: Level 3 – Changes greater than 2 fold from good quality signals that also pass a visual inspection. Level 2 - Changes greater than 2 fold from good quality signals that do not pass a visual inspection. Level 1 – Changes greater than 2 fold from low quality signals. Level 0 – No significant protein changes.

Fold changes: a semiquantitative value that represents the general trend of protein changes for experimental sample relative to control. 0/+ means no expression in control and the expression after treatment.