Table 2.

Altered protein expression of JAR cell with anti-Bag-1 antisense oligo nucleotide induced by hypoxia in the apoptosis pathways using PowerBlot.

Protein	Confidence level	(-) Under (+) Over	Fold change	Protein	Confidence level	(-) Under (+)Over	Fold change
Bcl-x	3	+	2.32	Ki-67	1	+	0/+
Caspase-3	3	+	3.75	Bad	0		1.08
Caspase-7	3	+	1.86	Bid	0		1.19
GST-p	3	+	3.31	Apaf-1	0		1.23
PTEN	3	+	1.91	Bcl-2	0		1.01
Smac/DIABLO	3	-	3.55	Pan-JNK/SAPK1-43kD	0		1.07
Hsp70-64kD	2	+	2.66	TRADD	0		1.22
Caspase-6	1	+	0/+	Bag-1			No exp.
JNK-phospho-specific	1	-	2.06

Fold change means a semiquantitative value that represents the general trend of protein changes for the experimental sample to control detected by using the Odyssey Infrared Imaging System. (+) means an increase of signal intensity, and (-) means a decrease of signal intensity after exposure to hypoxia.

Confidence levels are defined as: Level 3 – Changes greater than 2 fold from good quality signals that also pass a visual inspection. Level 2 - Changes greater than 2 fold from good quality signals that do not pass a visual inspection. Level 1 – Changes greater than 2 fold from low quality signals. Level 0 – No significant protein changes.

Fold changes: a semiquantitative value that represents the general trend of protein changes for experimental sample relative to control. 0/+ means no expression in control and the expression after treatment.

No exp. means no expression both in control and after treatment.