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. 2006 Dec 29;4(1):36–44. doi: 10.7150/ijms.4.36

Table 3.

Slope of standard curve and correlation coefficient of representative genes by Semiquantitative RT-PCR after 24 hours exposure to hypoxia.

Gene Slope Correlation coefficient
JNK1 -3.24 0.989
PTEN -3.59 0.993
Caspase 3 -3.25 0.996
GAPDH -3.53 0.999

mRNA was obtained after 24 hours exposure to hypoxia for JAR cells and JAR cells with Bag-1 Morpho/AS. Real-time semiquantitative RT-PCR was performed using an ABI 7500 RealTime PCR System. Then standard curves were obtained, and the slope and correlation coefficient were calculated for each gene.