Table 3.
Gene | Slope | Correlation coefficient |
---|---|---|
JNK1 | -3.24 | 0.989 |
PTEN | -3.59 | 0.993 |
Caspase 3 | -3.25 | 0.996 |
GAPDH | -3.53 | 0.999 |
mRNA was obtained after 24 hours exposure to hypoxia for JAR cells and JAR cells with Bag-1 Morpho/AS. Real-time semiquantitative RT-PCR was performed using an ABI 7500 RealTime PCR System. Then standard curves were obtained, and the slope and correlation coefficient were calculated for each gene.