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. 2006 Nov 17;73(2):630–636. doi: 10.1128/AEM.02164-06

FIG. 3.

FIG. 3.

Confocal scanning laser microscopic analysis showing effect of DOL and nanomolar concentration of QD conjugates in obtaining single-cell resolution of S. gordonii DL1 biofilms. Biofilms labeled with the general nucleic acid stain SYTO9 (green) or antibody conjugates (red): A, anti-DL1-Alexa Fluor 546 at 5 μg protein/ml; B, anti-DL1-QD655 at 5 μg protein/ml and 10 nM QD concentration; C, anti-DL1-QD655 at 10 μg protein/ml and 20 nM QD concentration; D, anti-DL1-QD655 at 15 μg protein/ml and 30 nM QD concentration with DOL of 3. Images are single optical sections.