Influence of culture conditions on the growth, production of pigment, and antibacterial activity of Phaeobacter strain 27-4 and Silicibacter sp. strain TM1040 in MB at 25°C. The data represent the mean of duplicate cultures (error bars represent standard deviations). (A) Growth was measured by plate counts. □, shaken culture of Phaeobacter strain 27-4; ○, static culture of Phaeobacter strain 27-4; ▿, shaken culture of Silicibacter sp. strain TM1040; ▵, static culture of Silicibacter sp. strain TM1040. (B) Pigment formation, as measured by absorbance at OD398. (C) Antibacterial activity represented by the zone of inhibition (in millimeters), as determined by using the well diffusion assay with V. anguillarum.