TABLE 1.
Comparison of E. coli strains for homolactate fermentationa
| Strain | Base | OD600d | Acetate (g/liter)d | Final concn (g/liter) for indicated agent
|
||||
|---|---|---|---|---|---|---|---|---|
| Lactate | Pyruvate | Acetate | Succinate | Ethanol | ||||
| YYC202 | NaOH | 32.3 | 1.4 | 75.1 | 9.1 | 0.1 | 4.4 | 0 |
| YYC202 | Ca(OH)2 | 22.9 | 5.1 | 90.0 | 2.6 | 1.8 | 7.3 | 0 |
| ALS961b | Ca(OH)2 | 4.5 | 7.2 | 14.1 | 7.1 | 7.0 | 0 | 0 |
| ALS974 | Ca(OH)2 | 26.0 | 5.1 | 92.0 | 2.1 | 0.3 | 2.6 | 0.6 |
| ALS974c | Ca(OH)2 | 30.6 | 5.0 | 138.1 | 1.5 | 0 | 2.3 | 0.3 |
a
Fermentor volumes were initially 1.5 liters. The two substrates glucose and acetate were fed until the OD600 reached about 30 during aerobic growth, and then 600 g/liter glucose was fed anaerobically to maintain 10 g/liter until the volume reached about 2.4 liters. Data represent means for two experimental runs.
b
Medium supplemented with 1.0 g/liter glutamate.
c
An initial volume of 1.0 liters and a more concentrated base were used.
d
At the end of the aerobic phase.