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. 2006 Nov 10;73(2):390–398. doi: 10.1128/AEM.02143-06

FIG. 2.

FIG. 2.

Effect of the rhdA mutation on rhodanese activity in P. aeruginosa PAO1. (A) Complementation analysis of P. aeruginosa PAO1 ΔrhdA with pUCPrhdA and pUCP4955-rhdA. The pUCP18 vector was used as a control. Bacteria were grown in LB medium at 37°C for 8 h before cell fractionation (see Materials and Methods). Specific rhodanese activity is reported for the cytoplasmic protein fraction. Results are the means from three independent experiments. Standard deviations are <5% of given values. (B) Immunoblot analysis of RhdA levels in cytoplasmic preparations (35 μg of total protein) from P. aeruginosa PAO1, PAO1 ΔrhdA, and the ΔrhdA mutant complemented with either pUCPrhdA or pUCP4955-rhdA. Purified recombinant RhdA (r-RhdA) (150 ng) was used as a control. Blots were hybridized with a mouse anti-RhdA polyclonal antiserum.