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. 2006 Nov 10;73(2):390–398. doi: 10.1128/AEM.02143-06

FIG. 4.

FIG. 4.

Effect of the rhdA mutation on P. aeruginosa growth under cyanogenic conditions. (A) Growth of wild-type P. aeruginosa PAO1-Nal (circles), PAO1-Rif ΔrhdA (squares), and PAO1-Rif ΔrhdA(pUCP4955-rhdA) (triangles) in NC-MM (white symbols) or C-MM (black symbols) at 37°C. Results are the means from triplicate growth assays. Standard deviations are <6% of the given values. The colorimetric detection of cyanide produced by individual strains at 12 h is shown in the inset. (B) CIs of P. aeruginosa PAO1-Rif ΔrhdA (squares) and PAO1-Rif ΔrhdA(pUCP4955-rhdA) (triangles) calculated at exponential (6 h) and stationary (18 h) phases in NC-MM (white symbols), C-MM (black symbols), and C-MM supplemented with 75 μM bovine methemoglobin (dashed line). CI values were calculated relative to wild-type P. aeruginosa PAO1-Nal (see Materials and Methods). Results are the means from at least three independent experiments. Standard deviations are shown.