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. 1999 Sep 14;96(19):10842–10847. doi: 10.1073/pnas.96.19.10842

Figure 4.

Figure 4

Analysis of anti-Tat and anti-p24 antibody responses by protein immunoblot. (Upper) Protein lysate from a normal T cell line (Nl TcL), lysate from a PBMC-derived T cell line engineered to overexpress the Tat protein (Tat TcL), and recombinant Tat protein (Rec Tat) were resolved by 12% SDS/PAGE, transferred to nitrocellulose, and probed with a rabbit polyclonal antiserum (RAB) to recombinant Tat, or with human antibody from a LTNP (DH). Protein markers (kDa) and expected migration of the Tat protein (filled arrow) are indicated. (Lower) Protein lysate from normal phytohemagglutinin-stimulated CD4+ PBMCs (Nl Tc), lysate from identical CD4+ cells infected with a dualtropic HIV-1 isolate (HIV Tc), recombinant human retinoblastoma 25-kDa fragment (Rec Control), and recombinant Tat (Rec TAT) were resolved by 12% SDS/PAGE, transferred to nitrocellulose, and probed with human antibodies from a LTNP (SF) or an IS (RR). Expected migration of p24 (open arrow) and Tat (filled arrow) proteins, and relative position of protein markers (kDa) are indicated.