Table 2.
Reproducibility of replicate spots within array
| Spike | SDa) | CVb) |
| sp 1 | 0.08 | 5.15% |
| sp 2 | 0.05 | 5.57% |
| sp 3 | 0.08 | 6.91% |
| sp 4 | 0.07 | 6.40% |
| sp 5 | 0.11 | 6.25% |
| sp 6 | 0.06 | 5.50% |
| sp 7 | 0.07 | 7.13% |
| sp 8 | 0.08 | 8.10% |
a)Standard deviation (SD) of M-values (M = log2R-log2G) was calculated for each series of 48 replicate spots within array. The averages of the SD of seven independent microarray experiments are shown. b) Coefficient of variation (CV) [(SD of M-values × 100)/mean of M-values] was calculated on the same sets of data and the averages of the seven independent microarray experiments are shown.