Table 4.

Variability of spike after excluding outlier values

	Before exclusion	After exclusion
Spike	CV a)	CV b)	Excluded c)
sp1	5.15%	4.85%	14% (1/7)
sp2	5.57%	5.57%	0
sp3	6.91%	6.31%	14% (1/7)
sp4	6.40%	6.40%	0
sp5	6.25%	4.80%	29% (2/7)
sp6	5.50%	5.50%	0
sp7	7.13%	6.48%	14% (1/7)
sp8	8.10%	6.47%	29% (2/7)

^a)Coefficient of variation (CV) [(SD of M-values × 100)/mean of M-values] was calculated for each series of 48 replicate spots within array before the outliers exclusion. The averages of the seven independent microarray experiments are shown. ^b) Coefficient of variation (CV) after the exclusion procedure. The CV was calculated as the mean of the remaining data after the filter. ^c) Percentage of experiments in which the single spike was excluded on a total of seven experiments. The number of experiments in which the specific spike was excluded out of the seven arrays analyzed is shown in parenthesis.