Figure 1.

Phylogenetic tree showing the overall relatedness of EF-hand-containing proteins in rice. Alignment of full-length protein sequences and phylogenetic analysis were performed as described in the "Methods" section. The numbers of EF hands predicted by InterProScan for each protein are shown as black blocks on the right with their heights proportional to their numbers of motif. With the exception of two proteins, all proteins indicated by the vertical line labelled "CaM & CML" at the right share more than 25% amino acid identity with OsCaM1 and were selected for further analyses. Positions of CBL and CPK members are also shown along the tree to emphasize their separation.