Figure 4.

Characteristics of EF hands in rice proteins. (a) Number of EF-hand-containing proteins containing 1, 2, 3 or 4 EF hands. (b) Residues in the EF hands #1-4 of OsCaMs compared with those of typical plant CaMs, vertebrate CaM (CaMv) and Saccharomyces cerevisiae CaM (CMD1p) using a consensus sequence of plant CaMs as a standard; identical residues in other sequences are indicated by a dash (-), and a gap introduced for alignment purposes is indicated by a dot (.). (c) Residues in Ca²⁺-binding loops in 32 OsCML proteins shown as the frequency at which an amino acid (shown at the left) is found in each position (shown at the top). The amino acids most frequently found are indicated by bold letters and shown below as a consensus sequence along with the positions of residues serving as Ca²⁺-binding ligands indicated in Cartesian coordinates. Bracketed residues are alternative residues frequently found in each position and "x" is a variety of amino acids. Residues serving as Ca²⁺-binding ligands are marked with asterisks (*).