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Impact of the cultivation temperature on the scFv production. Production of functional antibodies was analyzed by antigen binding ELISA of D1.3 scFvs of 50 μL culture supernatant after 24 h production in 100 mL scale. Mean values and standard deviations of data obtained from three different cultures are given. Antigens: 1 μg/well lysozyme or 1 μg/well control protein BSA. The D1.3 antibodies were detected using mAb mouse anti-His and goat anti-mouse IgGHRP (Fab specific).
