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. Author manuscript; available in PMC: 2007 Feb 13.
Published in final edited form as: J Biol Chem. 2006 Jan 30;281(12):7863–7872. doi: 10.1074/jbc.M509834200

Fig. 4.

Fig. 4

Effect of TAK1 inhibitor.

(A) 293 cells were transfected with empty vector or expression vectors for Flag-LMP1. 5Z-7-oxozeaenol was added to the culture medium every 12 hr after transfection and some cells were treated with 10 ng/ml TNF for 30 min prior to harvest. The activated form of JNK was detected with phospho-JNK antibody (P-JNK).

(B) 293 cells were transfected with NF-κB-dependent luciferase reporter and pAct-β-galactosidase plasmids, together with expression vectors for NIK or Flag-LMP1. 5Z-7-oxozeaenol was added to the culture medium at the concentrations indicated every 12 hr after transfection. Luciferase activity was determined and normalized to the levels of β-galactosidase activity. Stimulation relative to control transfection with empty vector is shown.