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. Author manuscript; available in PMC: 2007 Feb 13.
Published in final edited form as: J Biol Chem. 2006 Nov 1;281(52):39891–39896. doi: 10.1074/jbc.M608155200

Fig. 6.

Fig. 6

Effect of PP6 knockdown

(A) Two independent siRNAs targeted against PP6 (PP6-1 and PP6-2) or control non-targeting siRNA (Control) were transfected into the 293 IL-1RI cells. The efficiency of PP6 knockdown was examined at 48 hr post transfection. PP6 was detected with anti-PP6, and anti-PP2A and β-catenin blottings were used to assess specificity of the siRNAs.

(B) Cells were transfected with PP6 siRNA or control siRNA, and at 48 hr post transfection cells were treated with 5 ng/ml IL-1 for 1-3 min. Cell lysates were subjected to immunoblotting with anti-P-Thr-187, and anti-TAK1. IB, immunoblotting.

(C) A pool of siRNA for PP2A or control siRNA were transfected into the 293 IL-1RI cells. At 48 hr post transfection cells were treated with 5 ng/ml IL-1 for 1-3 min. Cell lysates were subjected to immunoblotting with anti-P-Thr-187, and anti-TAK1. IB, immunoblotting.