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. Author manuscript; available in PMC: 2007 Feb 13.
Published in final edited form as: Int Immunopharmacol. 2006 Nov 28;7(2):249–265. doi: 10.1016/j.intimp.2006.10.012

Fig. 1.

Fig. 1

The anti-CD4/anti-CD8 mAb cocktail induce antigen-specific tolerance in the DO11.10 CD4+ TCR transgenic mouse model. BALB/c mice were treated with 2.5 × 106 CD4+ KJ1-26+ cells isolated from BALB/c DO11.10 mice. On the same day they were immunized with either OVA, or β-gal, or no antigen, in combination with either the anti-CD4/anti-CD8 mAb cocktail or with no mAb, as indicated in panels a through d. All mice were bled on day 21 and OVA-specific (panel a) and β-gal-specific (panel b) antibody was measured in the serum by ELISA. On day 42 post-antigen-treatment, mice were boosted with either OVA or β-gal as indicated in panels c and d. 10 days after boosting OVA (panel c) and β-gal (panel d) specific antibodies were measured again. Data shown is mean ± SD and represents 2 experiments. ** indicates statistical significance to 0.001-0.009 and *** indicates statistical significance between 0.0001 - 0.0009. The Mann-Whitney test was used to determine statistical significance. n = 3 per group.