TABLE 2.
Primers used in multiplex amplification of MLGT assay
| Amplicon (size) | Primer | Region | Sequence (5′ to 3′)a |
|---|---|---|---|
| 1 (1,973 bp) | dnaE-F | ACC | GGATTTTCDCTTGGAGAAGCAG |
| dnaE-R | ACC | CRCCGACAACAGARCCCATAC | |
| 2 (1,782 bp) | hisS-F | AMI | CTTTCAGCRAGCGATTTATCAC |
| hisS-R | AMI | CGAACAACMGAAGCRGTCCC | |
| 3 (2,618 bp) | pdh-F | PDH | ACTTATCTGGCACGGACTTCC |
| pdh-R | PDH | TTTACGTACAGAAGGCATTGC | |
| 4 (2,479 bp) | hly-F2 | VGC | GAAGGAGAGTGAAACCCATG |
| mpl-R | VGC | CTCGCAACTTCYGGCTCAGC | |
| 5 (2,152 bp) | actA-F | VGC | CCAAYTGCATTACGATTAACC |
| plcB-R | VGC | CAAGCACATACCTAGAACCAC | |
| 6 (2,220 bp) | inlA-F2 | INL | ACGAAYGTAACAGACACGGTC |
| inlA-R2 | INL | CTACTTCTATTTACTAGCACG | |
| 7 (1,750 bp) | inlB-F2 | INL | AATCAAGGAGAGGATAGTGTG |
| inlB-R | INL | CTACCGGRACTTTATAGTAYG | |
| 8 (1,680 bp) | sigB-F | SIG | CATTACAACTTCCTGCCAAGC |
| sigB-R | SIG | GAACAAGTCCATCTGAATGCA | |
| 9 (2,052 bp) | lmo0300-F | LMO | GATTGGATTAAGTACGAGC |
| lmo0298-R | LMO | CTCTTATCAATGTGAAGGTGC |
a
See IUPAC codes for the definition of degenerate bases.