TABLE 2.
Results of preliminary screening of ORF clusters identified in SSH libraries
| RDI | ORF(s) represented in SSH libraries | ORF(s) targeted by primers | No. positive for LSPA-6 genotype
|
Genotype 111111 conserved? | |||
|---|---|---|---|---|---|---|---|
| 111111 (n = 10) | 222222 (n = 10) | 211111 (n = 5) | Other (n = 5) | ||||
| 14 | ECs0237-ECs0241 | ECs0238 and -9 | 10 | 0 | 5 | 0 | Yes |
| 16 | ECs0275-ECs0284 | ECS0281 | 10 | 0 | 5 | 0 | Yes |
| 27 | ECs0377-ECs0379 | ECs0377 | 10 | 10 | 5 | 5 | No |
| 52 | ECs0759-ECs0760 | ECs0760 | 10 | 9 | 5 | 5 | No |
| 67 | ECs1114-ECs1121 | ECs1120 | 10 | 10 | 5 | 5 | No |
| 69a | ECs1158-ECs1173 | ECs1164 | 8 | 0 | 3 | 0 | Yes |
| 69b | ECs1186-ECs1195 | ECs1191 | 7 | 0 | 0 | 0 | Yes |
| 69c | ECs1205-ECs1251 | ECs1223 | 10 | 2 | 3 | 0 | Yes |
| 72 | ECs1377-ECs1393 | ECS1382 | 10 | 0 | 4 | 0 | Yes |
| B1 | ECs1425 | ECs1425 | 10 | 10 | 5 | 5 | No |
| 78 | ECs1576-ECs1600 | ECs1588 and -9 | 8 | 0 | 0 | 0 | Yes |
| 82/83 | ECs1687-ECs1705 | ECs1698 | 10 | 0 | 3 | 0 | Yes |
| 91/92/93 | ECs1929-ECs1958 | ECs1954 | 10 | 0 | 0 | 0 | Yes |
| B2 | ECs1997-ECs1998 | ECs1998 | 10 | 10 | 5 | 5 | No |
| 126/127 | ECs2775-ECs2776 | ECs2775 | 10 | 10 | 5 | 5 | No |
| 153 | ECs2976-ECs2981 | ECs2979 | 10 | 0 | 2 | 0 | Yes |
| 274a | ECs4942-ECs4946 | ECs4943 | 3 | 5 | 0 | 3 | No |
| 274b | ECs4957-ECs4960 | ECs4960 | 1 | 4 | 0 | 2 | No |
| 274c | ECs4998-ECs5005 | ECs5000 | 10 | 9 | 5 | 5 | No |
| 286 | ECs5242-ECs5252 | ECs5250 | 9 | 0 | 4 | 0 | Yes |
| pO157-RTa | pO157, sopB-RT (p36) | Upstream of RT | 9 | 0 | 0 | 0 | Yes |
| pO157-katP | pO157, katP | katP | 9 | 10 | 5 | 5 | No |
| pO157-etpF | pO157, etpF | etpF | 10 | 10 | 5 | 5 | No |
| pOSAK1 | pOSAK1 | Unknown | 5 | 0 | 1 | 0 | No |
a
RT, putative reverse transcriptase.