TABLE 3.
Results of PCR screening to determine boundaries of CRDI in E. coli O157:H7 strains
| CRDI | ORF(s) targeted by primers | No. positive for LSPA-6 genotype
|
LSPA-6 genotype 111111 conserved? | |||
|---|---|---|---|---|---|---|
| 111111 (n = 10) | 222222 (n = 10) | 211111 (n = 5) | Other (n = 5) | |||
| 14 | ECs0234 and -5 | 10 | 10 | 5 | 5 | No |
| ECs0238 and -9 | 10 | 0 | 5 | 0 | Yes | |
| ECs0240 | 10 | 0 | 5 | 0 | Yes | |
| ECs0243 and -4 | 10 | 10 | 5 | 5 | No | |
| 16 | ECs0270 | 10 | 10 | 5 | 5 | No |
| ECs0271 and -2 | 10 | 0 | 5 | 0 | Yes | |
| ECs0276 | 10 | 0 | 3 | 0 | Yes | |
| ECS0281 | 10 | 0 | 5 | 0 | Yes | |
| ECs0282 and -3 | 10 | 10 | 5 | 5 | No | |
| ECs0284 | 10 | 10 | 5 | 5 | No | |
| 69a | ECs1158 | 10 | 10 | 5 | 5 | No |
| ECs1162 and -3 | 10 | 0 | 0 | 0 | Yes | |
| ECs1164 | 8 | 0 | 3 | 0 | Yes | |
| ECs1165 | 9 | 0 | 3 | 0 | Yes | |
| ECs1168 | 10 | 1 | 1 | 0 | Yes | |
| 69b | ECs1180 and -2 | 3 | 10 | 3 | 4 | No |
| ECs1186 and -8 | 3 | 0 | 0 | 0 | No | |
| ECs1191 | 7 | 0 | 0 | 0 | Yes | |
| ECs1192 and -4 | 10 | 9 | 3 | 3 | No | |
| 69c | ECs1217 and -8 | 10 | 10 | 5 | 5 | No |
| ECs1219 | 10 | 2 | 3 | 0 | Yes | |
| ECs1223 | 10 | 2 | 3 | 0 | Yes | |
| ECs1251 | 10 | 0 | 0 | 0 | Yes | |
| ECs1252 | 10 | 10 | 5 | 5 | No | |
| 72 | ECs1374 and -6 | 10 | 5 | 0 | 5 | No |
| ECs1377 | 10 | 0 | 3 | 0 | Yes | |
| ECS1382 | 10 | 0 | 4 | 0 | Yes | |
| ECs1389 and -90 | 10 | 0 | 5 | 0 | Yes | |
| ECs1394 and -5 | 10 | 10 | 5 | 5 | No | |
| 78 | ECs1573 and -4 | 8 | 10 | 5 | 5 | No |
| ECs1575 and -6 | 8 | 10 | 5 | 5 | No | |
| ECs1582 and -3 | 8 | 3 | 1 | 0 | Yes | |
| ECs1584 | 8 | 0 | 1 | 1 | Yes | |
| ECs1588 and -9 | 8 | 0 | 0 | 0 | Yes | |
| ECs1590 | 8 | 0 | 1 | 1 | Yes | |
| ECs1595 | 8 | 0 | 1 | 1 | Yes | |
| ECs1598 | 8 | 0 | 1 | 1 | Yes | |
| ECs1599 and -1600 | 8 | 0 | 0 | 0 | Yes | |
| 82/83 | ECs1687 | 10 | 10 | 5 | 5 | No |
| ECs1691 | 10 | 0 | 4 | 0 | Yes | |
| ECs1698 | 10 | 0 | 3 | 0 | Yes | |
| ECs1705 | 10 | 0 | 4 | 0 | Yes | |
| ECs1706 | 10 | 10 | 4 | 5 | No | |
| ECs1707 | 9 | 9 | 4 | 5 | No | |
| 91/92/93 | ECs1927 | 10 | 10 | 5 | 5 | No |
| ECs1931 and -2 | 10 | 0 | 0 | 0 | Yes | |
| ECs1937 to -40 | 10 | 0 | 0 | 0 | Yes | |
| ECs1945 | 10 | 2 | 0 | 1 | No | |
| ECs1953 | 10 | 0 | 0 | 0 | Yes | |
| ECs1954 | 10 | 0 | 0 | 0 | Yes | |
| ECs1957 | 10 | 0 | 0 | 0 | Yes | |
| 153 | ECs2979 | 10 | 0 | 2 | 0 | Yes |
| 286 | ECs5240 and -1 | 7 | 10 | 5 | 5 | No |
| ECs5242 | 9 | 0 | 5 | 0 | Yes | |
| ECs5250 | 9 | 0 | 4 | 0 | Yes | |
| ECs5252 | 9 | 0 | 5 | 0 | Yes | |
| ECs5253 | 10 | 10 | 5 | 5 | No | |
| ECs5254 to -6 | 10 | 10 | 5 | 5 | No | |
| PO157-RT | pO157, sopB | 9 | 10 | 5 | 5 | No |
| pO157, upstream of reverse transcriptase | 9 | 0 | 0 | 0 | Yes | |
| pO157, reverse transcriptase | 9 | 0 | 0 | 0 | Yes | |
| pO157, p36 | 9 | 10 | 5 | 5 | No | |