TABLE 1.
Percent protein sequence coverage of RDases and other Dehalococcoides proteins detected in three or more cultures
| RDase or protein (locus tag[s]) | % Protein sequence coverage
|
||||
|---|---|---|---|---|---|
| Strain 195
|
CBDB1 (pure culture) | KB1a | SRNLa | ||
| Pure culture | Mixed culture | ||||
| Reductive dehalogenases | |||||
| RDase putative PceA (DET0318, cbdbA1588) | 58 | 37 | 31 | ||
| RDase TceA (DET0079) | 55 | 57 | |||
| RDase (DET1559, cbdbA80) | 8 | 13 | |||
| RDase (DET1545, KB1 Rdha5) | 6 | 6 | |||
| RDase (cbdbA88) | 10 | ||||
| RDase (KB1 Rdha14, VcrA) | 21 | 31, 30 | |||
| Proteins detected in three or more strains | |||||
| Cochaperonin GroEL (DET1428, cbdbA1393) | 73 | 66 | 44 | 35, 58 | 25, 28 |
| Formate dehydrogenase (DET0187, cbdbA195) | 40 | 48 | 11 | 19, 47 | 2, 23 |
| Putative S-layer protein (DET1407, cbdbA1368) | 37 | 31 | 17 | 4, 37 | 4, 19 |
| Hypothetical protein (DET0754, cbdbA727) | 27 | 7 | 3 | 6, 8 | 0, 4 |
| Ketol acid reductoisomerase (DET0831, cbdbA811) | 47 | 36 | 10 | 17, 30 | |
| [Ni/Fe]-hydrogenase (DET0110, cbdbA129) | 45 | 30 | 6 | 3, 11 | |
| Myo-inositol-1-phosphate synthase (DET0979, cbdbA943) | 40 | 13 | 24 | 3, 6 | |
| Translation elongation factor (DET0997, cbdbA960) | 31 | 20 | 14 | 13, 16 | |
| GTPase domain protein (DET0589, cbdbA568) | 23 | 22 | 8 | 18, 21 | |
| Phospho-2-dehydro-3-deoxyheptonate aldolase (DET0468, cbdbA432) | 20 | 13 | 9 | 16, 16 | |
| Polyribonucleotide nucleotidyltransferase (DET0970, cbdbA932) | 9 | 8 | 4, 5 | ||
| ATP synthase F1, beta subunit (DET0564, cbdbA538) | 47 | 39 | 2, 5 | 10, 13 | |
| ATP synthase F1, alpha subunit (DET0562, cbdbA536) | 32 | 22 | 4, 8 | 2, 4 | |
| Hypothetical protein (DET0755, cbdbA728) | 31 | 21 | 10, 23 | 3, 19 | |
| SPFH domain protein (DET0848, cbdbA831) | 28 | 35 | 8, 8 | 33, 40 | |
| Serine protease (DET1285, cbdbA1217) | 26 | 8 | 3, 7 | 6, 6 | |
| Amino acid ABC transporter (DET0419, cbdbA371) | 22 | 10 | 0, 14 | 0, 19 | |
| ABC-type cobalamin/Fe3+ siderophore (DET0650, cbdbA633) | 22 | 4, 19 | 4, 36 | ||
| Hypothetical protein (DET0352, cbdbA294) | 14 | 0, 7 | 0, 9 | ||
a
Two values in one column entry indicate different coverages of locus tags provided at left, e.g., 31% coverage of KB1 Rdha14 and 30% coverage of VcrA in the SRNL mixed culture.