TABLE 2.
Sample location, date | No. of RNA copies/liter of raw sewageb | CC-RT-PCR U/liter of raw sewagec | No. of RNA copies/liter of treated effluent | CC-RT-PCR U/liter of treated effluent |
---|---|---|---|---|
Balaks, Nov. 1998 | 3.8 × 105 | 3.3 × 103 | 9.9 × 104 | 3.3 × 102 |
Balaks, Jan. 1999 | 8.7 × 106 | 3.3 × 103 | 1.1 × 105 | 3.3 × 102 |
Balaks, May 1999 | 8.7 × 105 | 3.3 × 102 | 6.2 × 105 | 3.3 × 101 |
El-Berka, Nov. 1998 | 1.1 × 105 | 3.3 × 103 | 1.1 × 103 | <3.3 × 100 |
El-Berka, July 1999 | 1.1 × 104 | <3.3 × 100 | NQ | <3.3 × 100 |
El-Berka, Aug. 1999 | 1.1 × 104 | <3.3 × 100 | NQ | <3.3 × 100 |
El-Berka, Sept. 1999 | 1.1 × 105 | 3.3 × 102 | NQ | <3.3 × 100 |
Zenin, Nov. 1998 | 9.9 × 105 | 3.3 × 103 | 1.1 × 103 | <3.3 × 100 |
Zenin, Dec. 1998 | 5.4 × 106 | 3.3 × 105 | NQ | <3.3 × 100 |
Zenin, Jan. 1999 | 6.2 × 105 | 3.3 × 102 | 1.1 × 104 | <3.3 × 100 |
Zenin, Feb. 1999 | 3.7 × 105 | 3.3 × 102 | 1.1 × 104 | <3.3 × 100 |
Zenin, Apr. 1999 | 1.1 × 105 | 3.3 × 101 | NQ | <3.3 × 100 |
Zenin, May 1999 | 1.1 × 105 | 3.3 × 101 | NQ | <3.3 × 100 |
Zenin, June 1999 | 1.1 × 104 | <3.3 × 100 | NQ | <3.3 × 100 |
Zenin, July 1999 | 1.1 × 104 | <3.3 × 100 | NQ | <3.3 × 100 |
Zenin, Oct. 1999 | 1.1 × 105 | 3.3 × 102 | 1.1 × 103 | <3.3 × 100 |
Reported values were determined by taking into consideration that 3-liter samples were concentrated to a final volume of 1 ml (3,000× concentration factor). NQ, nonquantifiable; the sample was positive only after Southern blot hybridization of the RT-PCR product.
The actual numbers of HAstV genogroup B copies in samples that also contained HAstV genogroup A were obtained by subtracting the number of genome copies estimated with primers A1/A2 (Table 1) from the numbers of genome copies estimated with primers A1, A1bis, and A2 internal.
The reciprocal endpoint dilution detected by CC-RT-PCR.