FIG. 1.

Recombinant murein hydrolases of the staphylococcal bacteriophage φ11. A. Schematic overview of the φ11 endolysin modules illustrating the constructs tested in this study. The φ11 endolysin features a modular design which consists of an N-terminal endopeptidase domain (endo), a central amidase domain (ami), and a C-terminal cell wall binding domain (CBD). The full-length and deletion constructs of the φ11 endolysin were overexpressed as six-His fusion proteins. B. Lytic activity of the staphylococcal bacteriophage φ11 endolysin domains.