Incubation of Cry1Aa and mutants with conditioned medium. Lane 1, Cry1Aa; lane 2, Cry1Aa incubated with conditioned medium; lane 3, Cry variant 1; lane 4, Cry variant 1 incubated with conditioned medium; lane 5, Cry variant 2; lane 6, Cry variant 2 incubated with conditioned medium; lane 7, Cry variant 4; lane 8, Cry variant 4 incubated with conditioned medium; lane 9, Cry variant 5; lane 10, Cry variant 5 incubated with conditioned medium. Numbers at the left are molecular masses (in kilodaltons).