TABLE 3.
Clone | Loop | Loop sequence |
---|---|---|
WT | 1 | CAT AGA GGC TTT |
H R G F | ||
2 | AGA AGA ATT ATA CTT GGT TCA GGC CCA AAT AAT CAG GAA | |
R R I I L G S G P N N Q E | ||
3 | AGC CAA GCA GCT GGA GCA GTT TAC ACC | |
S Q A A G A V Y T | ||
1 | 1 | GAG CGG GGG GTG |
E R G V | ||
2 | GGG GCC GCG GCC CAG CGG GGG TAC GGG TTC GCC TGC ACG | |
G A A A Q R G Y G F A C T | ||
3 | CCG TTG ACG GGG AAG GGG GCG TCG | |
P L T G K G A S | ||
2 | 1 | ACG ACC GGC CTC AAC |
T T G L N | ||
2 | TTG GAC AAC AGC TGG CGG GAG GAG AAG GGG GTG CGG GGC | |
L D N S W R E E K G V R G | ||
3 | TTC CAG GCG AGC CTC AGC TGG CAC AAG GCG | |
F Q A S L S W H K A | ||
4 | 1 | AAG GTG GAG CTG CAG |
K V E L Q | ||
2 | AAG TTG ATG TGG GGC TCC CTG GGC GAG GTG CAG CGG TGC | |
K L M W G S L G E V Q R C | ||
3 | CGG GGG GCC GCG CGG TAC TTG GTG | |
R G A A R Y L V | ||
5 | 1 | GAG CGC CTC |
E R L | ||
2 | GCC TGC CCG TCG GTG CCG CAG ATC TCG ATG ATC TCG TAC GGC | |
A C P S V P Q I S M I S Y G | ||
3 | CTG GCG GGC GCC GTA AAA CAG | |
L A G A V K Q |
The sequences of the wild-type (WT) loops are shown for comparison. Mutant loops show variations in sequence and length from the original sequences.