TABLE 1.

Fatty acid composition of total lipids in S. cerevisiae FAD2 transformants grown at different temperatures^a

Strain	Temp (°C)	Fatty acid composition (mol%)b						UIc	Total wt of fatty acidsd
		16:0	16:1	16:2	18:0	18:1	18:2
trp1 strains
W303-1A	30	20 ± 0.5	34 ± 1.2	UDe	11 ± 0.3	36 ± 1.4	UD	0.69 A	21
	15	20 ± 0.1	35 ± 0.7	UD	8.9 ± 0.2	37 ± 0.6	UD	0.71 A	22
W303FAD2-1	30	20 ± 0.4	30 ± 1.4	1.6 ± 0.4	11 ± 1.7	29 ± 0.6	8.0 ± 0.1	0.79 B	18
	15	20 ± 1.3	23 ± 1.1	6.0 ± 0.3	12 ± 0.9	18 ± 0.2	20 ± 2.8	0.94 C	26
W303FAD2-3	30	22 ± 0.8	27 ± 0.8	5.2 ± 0.0	12 ± 1.1	16 ± 0.4	19 ± 1.0	0.90 C	20
	15	20 ± 0.2	27 ± 0.5	5.1 ± 0.1	11 ± 0.1	15 ± 0.2	22 ± 0.6	0.97 C	26
trp1 TRP1 strains
W303-1A	30	21 ± 0.5	31 ± 0.5	UD	11 ± 1.6	37 ± 1.4	UD	0.68 A	39
	15	19 ± 0.9	39 ± 0.4	UD	9.5 ± 1.4	33 ± 0.4	UD	0.72 AB	49
W303FAD2-1	30	20 ± 0.9	30 ± 0.9	UD	11 ± 0.7	33 ± 0.5	6.0 ± 0.9	0.74 B	36
	15	19 ± 0.3	27 ± 0.6	7.2 ± 1.2	11 ± 0.2	16 ± 1.9	20 ± 1.2	0.97 C	45
W303FAD2-3	30	22 ± 0.9	23 ± 2.8	5.4 ± 1.7	12 ± 0.8	16 ± 3.3	21 ± 2.7	0.92 C	34
	15	19 ± 0.5	27 ± 2.0	6.6 ± 1.3	11 ± 1.9	16 ± 3.5	20 ± 1.5	0.96 C	48

^aTryptophan auxotrophic (trp1) and prototrophic (trp1 TRP1) cells of the S. cerevisiae W303-1A wild-type strain expressing the sunflower genes FAD2-1 and FAD2-3 were analyzed.

^bValues are the means ± standard errors of the means for at least three independent experiments with duplicate determinations of fatty acid composition.

^cUI, unsaturation index. The unsaturation index was defined as follows: [(percentage of 16:1 + percentage of 18:1) + 2(percentage of 16:2 + percentage of 18:2)]/100. Values with different letters are significantly different at a P of 0.01 (analysis of variance).

^dIn micrograms per milligram of cells (dry weight).

^eUD, undetected.