TABLE 2.
Growth of FAD2 transformants at different temperaturesa
| Strain | Doubling time (h) atb:
|
||
|---|---|---|---|
| 30°C | 15°C | 10°C | |
| W303-1A | 2.2 ± 0.1 | 11.5 ± 0.2 | 89 ± 5.3 |
| W303FAD2-1 | 1.7 ± 0.1 | 13.3 ± 0.2 | 107 ± 5.2 |
| W303FAD2-3 | 1.7 ± 0.1 | 12.8 ± 0.1 | 107.3 ± 12 |
a
Tryptophan auxotrophic (trp1) cells of the S. cerevisiae W303-1A wild-type strain expressing the sunflower genes FAD2-1 and FAD2-3 were analyzed. Cells were grown in liquid SD medium.
b
Values represent the means ± standard errors of the means for at least two independent experiments.