Table 3.
Area fraction of vacuoles, number of apoptotic cells, mean vascular density, vascular diameter and ratio central/peripheral vessel density and vessel diameter in controls and in tumors treated with hyperoxia. Values are given as means ± SD.
|
Control 1 bar, 21% O2 pO2 = 0.21 |
Test 1 bar, 100% O2 pO2 = 1.0 |
Test 1.5 bar, 100% O2 pO2 = 1.5 |
|
| Area fraction vacuoles "empty spaces" | 0.01 ± 0.04 | 0.11 ± 0.05 *** | 0.21 ± 0.02 *** |
| Apoptotic cells (% of total cells) | 61% ± 6.4 | 85% ± 7.3 *** | 88% ± 5.0 *** |
| Mean vascular density (number of vessels/mm2) | |||
| Centrally | 98 ± 18.6 | 50.1 ± 17.4*** | 69.8 ± 18.5* |
| Periphery | 79.5 ± 19.2 | 38.5 ± 19.3*** | 51.8 ± 26.8* |
| Vascular diameter (μm) | |||
| Centrally | 19.1 ± 4.9 | 23.1 ± 9.1 ** | 23.2 ± 10.1** |
| Periphery | 10.9 ± 3.9†† | 12.5 ± 4.7 *†† | 23.9 ± 10.0 *** |
| Ratio central/peripheral vessel density | 1.2 ± 0.7 | 1.6 ± 1.3 | 1.5 ± 1.1* |
| Ratio central/peripheral vessel diameter | 1.9 ± 0.8 | 2.0 ± 1.2 | 1.1 ± 0.6*** |
*P < 0.05, **P < 0.01, ***P < 0.001 vs control (1 bar, air)
††P < 0.01 vs centrally