Table 1.
Protein expression of stem cell markers and others in OGCTs
| HISTOLOGY of core | N | N bilat | OCT-3/4 | NANOG | KIT | AP-2γ | TSPY | MAGE-A4 | NY-ESO-1 | AMH | PLAP | HCG | AFP | |||||||||||
| OVARIAN TISSUES | ||||||||||||||||||||||||
| Foetal GW 13–18 | 4 | OCT-3/4 in foetal ovary: Ref [21] | Neg | KIT in foetal ovary: Ref [31] | AP-2γ in foetal ovary: Ref [27] | Neg | Oogonia++ | Oogonia+/- | AMH in foetal ovary: Ref [19] | n.a. | n.a. | n.a. | ||||||||||||
| Foetal GW 20–24 | 4 | Neg | Neg | Oogonia++ | Oogonia+/- | n.a. | n.a. | n.a. | ||||||||||||||||
| Foetal GW 34–40 | 4 | Neg | Neg | Oogonia++ | Oogonia+/- | n.a. | n.a. | n.a. | ||||||||||||||||
| DG | 17 | 4 | ++/+- | 80% | ++ | 67% | ++/+ | 80%** | ++/+ | 33% | +- | 13% | ++/+- | 40% | - | ++/+- | 100% | ++ | 7% | ++ | 7% | |||
| DG (w/GB)¤ | 8 | 4 | ++/+- | 75% | ++/+ | 38% | ++ | 88%** | +/+- | 38% | ++/+ | 63% | + | 13% | - | - | ++/+- | 100% | - | - | ||||
| GB | 2 | 1 | ++/+ | 100% | + | 50% | ++/+ | 100%** | - | ++ | 50% | - | - | - | ++ | 100% | - | - | ||||||
| EC | 1 | - | + | 100% | - | - | - | - | - | - | ++ | 100% | - | - | ||||||||||
| YST | 14 | 1 | + | 8% | + | 15% | +/+- | 15% | - | - | - | - | - | ++/+- | 46% | - | ++/+- | 77% | ||||||
| IT | 17 | - | - | - | - | - | - | - | - | - | - | +- | 6% | |||||||||||
| CC | 1 | - | - | - | - | - | - | - | - | - | ++ | 100% | - | |||||||||||
| SCC | 8 | - | - | - | - | - | - | - | - | - | - | - | ||||||||||||
| NON-OVARIAN TISSUES | ||||||||||||||||||||||||
| Adult normal testis | 3 | - | +- | (unsp) | + | 100%† | - | ++ | 100%|| | + | 100%|| | + | 33%†† | ++ | 100%‡ | - | - | - | ||||||
| CIS testis | 2 | ++ | 100% | ++ | 100% | ++ | 100% | ++ | 100% | ++ | 100% | - | - | - | n.a. | n.a. | n.a. | |||||||
| Seminoma testis | 2 | ++ | 100% | ++ | 100% | ++ | 100% | ++ | 50% | ++ | 100% | - | - | - | ++ | 100% | - | - | ||||||
| EC/TER testis | 2 | ++ | 100% | ++ | 100% | + | 50% | ++/+ | 100% | + | 100% | - | - | - | ++/+ | 100% | ++ | 100% | + | 50% | ||||
| YST testis | 1 | ++ | 100% | ++ | 100% | - | - | - | - | - | - | + | 100% | ++ | 100% | ++ | 100% | |||||||
| Colon cancer | 2 | - | - | - | - | - | - | - | - | - | - | - | ||||||||||||
| Prostate cancer | 2 | - | - | - | - | - | - | - | - | - | - | - | ||||||||||||
| Normal brain | 1 | - | - | - | - | - | - | - | - | - | - | - | ||||||||||||
| Normal kidney | 1 | - | - | - | (unsp) | - | (unsp) | +- | (unsp) | - | - | - | - | - | - | |||||||||
| Normal liver | 2 | - | - | - | (unsp) | - | (unsp) | - | - | - | - | - | - | - | ||||||||||
Staining intensity is marked by '++'/'+'/'+-'/'-' followed by the percentage of positive tissue samples. Staining intensity = '++': >50%, of tumour cells stained; '+': staining in approximately 20–50% of tumour cells; '+-': few cells stained; '-': no positive cells detected. For each tumour type the range of staining intensities are divided with '/'. Symbols = †: staining in spermatocytes; ||: staining in spermatocytes and spermatogonia; ††: staining in spermatogonia; ‡: staining in pre-pubertal Sertoli cells; **: all bilateral DG or GB stained '++' with KIT; ¤: DG tissue from patients with diagnosis DG with GB. Abbreviations: OGCT = ovarian germ cell tumour; GW = gestational week; DG = dysgerminoma; GB = gonadoblastoma; EC = embryonal carcinoma; YST = yolk sac tumour; IT = immature teratoma; CC = choriocarcinoma; SCC = small cell carcinoma; CIS = carcinoma in situ; TER = teratoma; N = number; bilat = bilateral; n.a. = not analysed; ref = reference; unsp = unspecific.