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. 2007 Jan 29;7:3. doi: 10.1186/1472-6882-7-3

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Copyright © 2007 Ong and Tan; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Radioligand displacement assay of E. ulmoides (EU) extract. Tritiated testosterone was incubated with transfected Hela cells transiently expressing AR protein. Concentration-dependent competitive displacement of tritiated testosterone by androgenic E. ulmoides (EU) extract (1c.f. = 50 ng/ml treatment medium) was measured by the scintillation count of the bound radioisotopic testosterone to AR protein. DHT (nM) and cortisol (nM) acted as positive and negative controls respectively. Data are mean ± SE of four replicates.